Laminar Flow Hoods and Biosafety Cabinets

CRX 105 offers a foundational understanding of critical equipment like laminar airflow workstations (LAFWs), biosafety cabinets (BSCs) across all classes, and compounding aseptic containment/isolator enclosures (CACIs/CAIs). The curriculum covers the principles of airflow dynamics, filtration systems, and containment strategies essential for various applications, including sterile compounding, research, and quality control. Students will learn to identify different equipment types, understand their operational modes, interpret alarm systems, and perform routine checks. The course emphasizes safety protocols, regulatory considerations, and the critical role these devices play in protecting personnel, products, and the environment. Through a blend of theoretical instruction and hands-on laboratory exercises, technicians will develop the competencies required for effective operation and basic troubleshooting.

InstructorJacob Calhoun

Prerequisites

CRX 101

Course outline

Lectures, virtual labs, and graded assignments — completed in your browser.

01Lesson 1: Introduction to Primary Engineering Controls and Controlled Environmentslecture

02Lesson 2: Principles of Airflow Dynamics and HEPA Filtrationlecture

03Lesson 3: Laminar Airflow Workstations (LAFWs): Design and Operationlecture

04Hands-on Lab: LAFW Setup, Operation, and Basic Airflow Verificationlab

05Lesson 5: Biosafety Cabinet (BSC) Fundamentals: Classification and Purposelecture

06Lesson 6: Biosafety Cabinet (BSC) Class I and Class II: Type A Serieslecture

07Lesson 7: Biosafety Cabinet (BSC) Class II: Type B Series and Class IIIlecture

08Hands-on Lab: BSC Class II Operation, Contamination Control, and Alarm Responselab

09Lesson 9: Compounding Aseptic Containment Isolators (CACIs) and Isolators (CAIs)lecture

10Lesson 10: Airflow Patterns, Containment Integrity, and Monitoring Systemslecture

11Hands-on Lab: Personal Protective Equipment (PPE) Donning/Doffing and Aseptic Technique in PECslab

12Lesson 12: Decontamination, Cleaning Protocols, and Emergency Procedureslecture

13Lesson 13: Understanding Alarms, Indicators, and Routine Performance Checkslecture

14Capstone Service Project: Comprehensive PEC Inspection and Operational Readiness Assessmentassignment

Syllabus

["Week 1: Lesson 1: Introduction to Primary Engineering Controls and Controlled Environments","Week 2: Lesson 2: Principles of Airflow Dynamics and HEPA Filtration","Week 3: Lesson 3: Laminar Airflow Workstations (LAFWs): Design and Operation","Week 4: Hands-on Lab: LAFW Setup, Operation, and Basic Airflow Verification","Week 5: Lesson 5: Biosafety Cabinet (BSC) Fundamentals: Classification and Purpose","Week 6: Lesson 6: Biosafety Cabinet (BSC) Class I and Class II: Type A Series","Week 7: Lesson 7: Biosafety Cabinet (BSC) Class II: Type B Series and Class III","Week 8: Hands-on Lab: BSC Class II Operation, Contamination Control, and Alarm Response","Week 9: Lesson 9: Compounding Aseptic Containment Isolators (CACIs) and Isolators (CAIs)","Week 10: Lesson 10: Airflow Patterns, Containment Integrity, and Monitoring Systems","Week 11: Hands-on Lab: Personal Protective Equipment (PPE) Donning/Doffing and Aseptic Technique in PECs","Week 12: Lesson 12: Decontamination, Cleaning Protocols, and Emergency Procedures","Week 13: Lesson 13: Understanding Alarms, Indicators, and Routine Performance Checks","Week 14: Capstone Service Project: Comprehensive PEC Inspection and Operational Readiness Assessment"]